Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Inositol Size:500g Dilute 50 ml MOPS-SDS 20X

Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer

4°C Water KF 0

2 at 25°C Suspend 20 gram powdered medium in 1 litre distilled water

Process Biochemistry

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Inositol Size:500g Dilute 50 ml MOPS-SDS 20XInositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature