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Tryptone 10 Yeast extract 5 NaCl 8 Final pH 7
Final concentration of 50mM MES
2 at 25°C Suspend 42
Protein Gel Electrophoresis
LBM-Broth Lennox Size:250g Tryptone 10 Yeast extract 5LBM Broth Low Salt For propagation of of lambda phages in Escherichia coli. In LBM media Mg2 is added to enhance absorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 5 MgSO4 anhydrous 0. 98 Final pH 7. 0 0. 2 at 25C Suspend 21 gram powdered medium in 1 litre distilled water Store dry at room temperature
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