Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Pantothenate Size:1000g 05 M 30

05  M  30

Yeast extract 10 Peptone 20 Maltose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

Addition of magnesium and calcium increases the growth of the recombinant cells

20 Heavy Metals <5 ppm pKa at 20°C 7

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Pantothenate Size:1000g 05 M 30Calcium pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium pantothenate or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store