Yeast Nitrogen base without Amino acids and without Copper Size:500g Pyridoxine HCl is omitted from

Pyridoxine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of pyridoxine or to replace this vitamin

Kanamycin is used as a selective agent in combination with the incorporation of the NPT II (AH3’) gene in plant tissue and microbial cells

2 at 25°C Suspend 38 gram powdered medium in 1 litre distilled water

186 Agar 15 Final pH 7

Yeast Nitrogen base without Amino acids and without Copper Size:500g Pyridoxine HCl is omitted fromCupric sulphate, CuSO4. 5H2O, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper or to replace this element. Suspend 6. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature