Neidhardt Basal Salt Mixture, Glucose 20 g/L Size:658g (20L) 1 g/L SDS 69

1  g/L SDS 69

coli is grown to late log phase in LB Broth

Potency  Approximately 640 µ/mg Store dry at 2-8°C

To avoid errors due to this reserve of nitrogen it is advised to make two serial transfers in the medium

Neidhardt Basal Salt Mixture, Glucose 20 g/L Size:658g (20L) 1 g/L SDS 69Containing all micro and macro salt, buffers and glucose. A mixture of Neidhardt basal salt mixture in a concentration of 32. 9 grams l with Neidhardt supplement mixture in a concentration of 3. 1 grams l. Suspend 32. 9 grams of Neidhardt basal salt mixture plus 3. 1 grams of Neidhardt supplement mixture in one litre of distilled water. Sterilisation by means of filtration. After dissolving a slight precipitation might occur. It is not clear what this