Yeast Nitrogen Base without Amino Acids and without Phosphate Size:500g Dilute 50 ml MOPS-SDS 20X

Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer

15 gram in 1 litre distilled water for 1X supplements

To make YEPG-Medium add 38 ml glycerol per litre YEP medium

Yeast Nitrogen Base Vitamin Free is based upon the formulation of Yeast Nitrogen base except that all vitamins are omitted

Yeast Nitrogen Base without Amino Acids and without Phosphate Size:500g Dilute 50 ml MOPS-SDS 20XThe absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. Suspend 5. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature