SIH Medium Size:250g Yeast extract 10 Peptone 20

Yeast extract 10 Peptone 20 Maltose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

Final concentration of 50mM MES

4 Riboflavin 0

Methionine

SIH Medium Size:250g Yeast extract 10 Peptone 20SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic