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5 X sterile stock solution
0 Beef Extract 5
2 KH2PO4 1
Tryptone 10 Yeast extract 5 (NH4)2SO4 3
LBM-Agar Miller Size:250g 5 X sterile stock solutionLBM Agar Low Salt In LBM media Mg2 is added to enhance absorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Agar 15 Final pH 7. 0 0. 2 at 25C Suspend 41 gram powdered medium in 1 litre distilled water Store dry at room temperature
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