Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:1000g Malt extract is prepared from

Malt extract is prepared from Malt by extracting the soluble products from sprouted grain

2 Thiamine 3

Due to its carbohydrate content

35 mM) of KCl is added

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:1000g Malt extract is prepared fromThiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room