Yeast Nitrogen Base without Amino Acids and without Phosphate, supplemented with KCl Size:250g minus Cysteine and without Glutamic

minus Cysteine and without Glutamic acid

Peptone 10 Yeast Extract 1 Glucose 10 KH2PO4 1

is based on the Amino acids present in FM Minimal medium

Dilute 100ml TBE Buffer 10X into 900ml deionised water to make 1 litre of TBE Buffer

Yeast Nitrogen Base without Amino Acids and without Phosphate, supplemented with KCl Size:250g minus Cysteine and without GlutamicThe absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. By omitting Potassium DiHydrogen Phosphate (KH2PO4), not only Phosphate (PO4 3) but also Potassium (K ) is omitted. As a result yeasts are not only limited in Phosphate, but also in Potassium. This might cause problems in osmo molarity and